Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
J Agric Food Chem. 2011 Aug 10;59(15):8086-94. doi: 10.1021/jf201158k. Epub 2011 Jul 7.
Alkylamides are a class of compounds present in plants of the genus Echinacea (Asteraceae), which have been shown to have high bioavailability and immunomodulatory effects. Fast analysis to identify these components in a variety of products is essential to profile products used in clinical trials and for quality control of these products. A method based on ultrafast liquid chromatography (UFLC) coupled with diode array detection and electrospray ionization mass spectrometry was developed for the analysis of alkylamides from the roots of Echinacea angustifolia (DC.) Hell., Echinacea purpurea (L.) Moench, and commercial dietary supplements. A total of 24 alkylamides were identified by LC-MS. The analysis time for these components is 15 min. Compared to the alkylamide profiles determined in the Echinacea root materials, the commercial products showed a more complex profile due to the blending of root and aerial parts of E. purpurea. This versatile method allows for the identification of alkylamides in a variety of Echinacea products and presents the most extensive characterization of alkylamides in E. angustifolia roots so far.
酰胺是存在于紫菀科松果菊属（Echinacea）植物中的一类化合物，已被证明具有较高的生物利用度和免疫调节作用。快速分析以鉴定各种产品中的这些成分对于分析临床试验中使用的产品和这些产品的质量控制至关重要。建立了一种基于超快速液相色谱（UFLC）结合二极管阵列检测和电喷雾电离质谱的方法，用于分析狭叶松果菊（Echinacea angustifolia（DC.）Hell.）、紫松果菊（Echinacea purpurea（L.）Moench）根和商业膳食补充剂中的酰胺。通过 LC-MS 鉴定了 24 种酰胺。这些成分的分析时间为 15 分钟。与在松果菊根材料中确定的酰胺图谱相比，由于紫松果菊的根和地上部分的混合，商业产品显示出更复杂的图谱。这种多功能方法可用于鉴定各种松果菊产品中的酰胺，并迄今为止对狭叶松果菊根中的酰胺进行了最广泛的表征。
