Li Chen, Xu Feng, Xie De-Mei, Jing Yu, Shang Ming-Ying, Liu Guang-Xue, Wang Xuan, Cai Shao-Qing
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China.
Molecules. 2014 Apr 17;19(4):4857-79. doi: 10.3390/molecules19044857.
Traditional Chinese Medicine (TCM) nasal therapy has been utilized to treat numerous diseases for over two millennia. It has many advantages compared with other routes. In this article, headspace-solid phase microextraction-gas chromatography-mass spectrometry and high performance liquid chromatography-atmospheric pressure chemical ionization-ion trap-time of flight-multistage mass spectrometry were applied for the first time to analyze the absorbed constituents in rabbit plasma and cerebrospinal fluid (CSF) after intranasal administration of Asari Radix et Rhizoma (AR). In total, 47 absorbed AR constituents including 14 monoterpenes, 10 phenylpropanoids, four benzene derivatives, two alkanes, nine N-alkylamides and eight lignans were tentatively identified in the rabbit plasma and CSF. Thirty-three absorbed constituents are found to have different bioactivities related to the pharmacological actions of AR through bibliography data retrieval. These indicated that many types of constituents of TCM can be absorbed at the nasal cavity into both rabbit blood and CSF. This is the first study to explore the absorption of AR, and comprehensively analyze the absorbed constituents after intranasal administration of TCM. These findings extend our understanding of the effective substances of AR, and inspire us to make a hypothesis on the mechanism of additive effect of multiple constituents of TCMs, which is very worthy of further investigation.
两千多年来，中医鼻疗法一直被用于治疗多种疾病。与其他给药途径相比，它有许多优点。在本文中，首次应用顶空固相微萃取-气相色谱-质谱联用技术和高效液相色谱-大气压化学电离-离子阱-飞行时间-多级质谱联用技术，分析兔鼻腔给予细辛（AR）后血浆和脑脊液（CSF）中的吸收成分。在兔血浆和脑脊液中初步鉴定出47种细辛吸收成分，包括14种单萜类、10种苯丙素类、4种苯衍生物、2种烷烃、9种N-烷基酰胺和8种木脂素。通过文献数据检索发现，33种吸收成分具有与细辛药理作用相关的不同生物活性。这些结果表明，中药的多种成分可经鼻腔吸收进入兔血液和脑脊液。这是首次探索细辛吸收情况，并对中药鼻腔给药后的吸收成分进行综合分析的研究。这些发现拓展了我们对细辛有效物质的认识，并启发我们对中药多成分相加作用机制提出假设，非常值得进一步研究。
