釉基质蛋白对人牙槽骨细胞增殖、分化和附着的影响。
Effects of enamel matrix proteins on proliferation, differentiation and attachment of human alveolar osteoblasts.
机构信息
Department of Periodontology, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, China.
出版信息
Cell Prolif. 2011 Aug;44(4):372-9. doi: 10.1111/j.1365-2184.2011.00762.x.
OBJECTIVES
Enamel matrix proteins (EMPs) have been demonstrated to promote periodontal regeneration. However, effects of EMPs on human alveolar osteoblasts (hAOBs), up to now, have still been unclear. The purpose of this study was to investigate influence of EMPs on proliferation, differentiation and attachment of hAOBs in vitro.
MATERIALS AND METHODS
EMPs were extracted using the acetic acid method, hAOBs were obtained and cultured in vitro. Cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of osteogenic markers and cell attachment were measured in the absence and in the presence of EMPs (50, 100 and 200 μg/ml).
RESULTS
EMPs increased proliferation of hAOBs; however, they inhibited ALP activity and mRNA expression of osteogenic markers (collagen I, ALP, runt-related protein 2, osteocalcin, bone sialoprotein and osteopontin). Meanwhile, EMPs hindered hAOBs' attachment. These effects occurred in EMPs concentration-dependent manner.
CONCLUSIONS
These results indicate that EMPs may inhibit osteoblastic differentiation and attachment to prevent ankylosis and allow other cell types to regenerate periodontal tissues.
目的
已有研究证实，釉基质蛋白（EMPs）可促进牙周组织再生。然而，EMPs 对人牙槽骨成骨细胞（hAOBs）的影响目前仍不清楚。本研究旨在探讨 EMPs 对体外培养的 hAOBs 增殖、分化和黏附的影响。
材料与方法
采用醋酸法提取 EMPs，体外分离培养 hAOBs。在 EMPs（50、100 和 200μg/ml）存在和不存在的情况下，检测细胞增殖、碱性磷酸酶（ALP）活性、成骨标志物的 mRNA 表达和细胞黏附。
结果
EMPs 可促进 hAOBs 的增殖，但抑制 ALP 活性和成骨标志物（I 型胶原、ALP、Runt 相关转录因子 2、骨钙素、骨涎蛋白和骨桥蛋白）的 mRNA 表达。同时，EMPs 还抑制 hAOBs 的黏附，且这种作用呈浓度依赖性。
结论
这些结果表明，EMPs 可能通过抑制成骨细胞分化和黏附来防止牙周组织的附着性骨丧失，从而允许其他细胞类型再生牙周组织。
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