高通量 DNA 分离方法用于检测植物和昆虫样本中的韧皮部杆菌。
High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples.
机构信息
Texas AgriLife Research, Stephenville, TX, USA.
出版信息
J Microbiol Methods. 2011 Sep;86(3):310-2. doi: 10.1016/j.mimet.2011.06.007. Epub 2011 Jun 13.
PMID:21703312
Abstract
We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.
摘要
我们报告了一种从昆虫和植物样本中分离 DNA 的经济高效、高通量方法，目的是检测韧皮部坏死病菌（Xylella fastidiosa）感染。现有的方法往往会共纯化 DNA 聚合酶的抑制剂，限制了其在基于 PCR 的检测中的应用。与市售试剂盒相比，该方法以更低的成本提供了更高的病原体检测效果。
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