Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
Chemosphere. 2011 Sep;84(11):1636-41. doi: 10.1016/j.chemosphere.2011.05.024. Epub 2011 Jun 23.
The uptake of Se (VI) by two aquatic plants, Myriophyllum spicatum L. and Ceratophyllum demersum L., and its effects on their physiological characteristics have been studied. Plants were cultivated outdoors under semi-controlled conditions and in two concentrations of Na selenate solution (20 μg Se L(-1) and 10 mg Se L(-1)). The higher dose of Se reduced the photochemical efficiency of PSII in both species, while the lower dose had no effect on PSII. Addition of Se had no effect on the amounts of chlorophyll a and b. The concentration of Se in plants grown in 10 mg Se L(-1), averaged 212 ± 12 μg Seg(-1) DM in M. spicatum (grown from 8-13 d), and 492 ± 85 μg Se g(-1) DM in C. demersum (grown for 31 d). Both species could take up a large amount of Se. The amount of soluble Se compounds in enzyme extracts ranged from 16% to 26% in control, and in high Se solution from 48% to 36% in M. spicatum and C. demersum, respectively. Se-species were determined using HPLC-ICP-MS. The main soluble species in both plants was selenate (∼37%), while SeMet and SeMeSeCys were detected at trace levels.
研究了两种水生植物（狐尾藻和金鱼藻）对六价硒（Se(VI)）的吸收及其对其生理特性的影响。植物在半控制条件下和两种亚硒酸钠溶液浓度（20 μg Se L(-1)和 10 mg Se L(-1)）下进行室外培养。较高剂量的 Se 降低了两种物种的 PSII 光化学效率，而低剂量的 Se 对 PSII 没有影响。添加 Se 对叶绿素 a 和 b 的含量没有影响。在 10 mg Se L(-1)中生长的植物中，Se 的浓度在狐尾藻中平均为 212 ± 12 μg Se g(-1) DM（生长 8-13 天），在金鱼藻中为 492 ± 85 μg Se g(-1) DM（生长 31 天）。两种植物都可以吸收大量的 Se。在对照中，酶提取物中可溶性 Se 化合物的含量范围为 16%-26%，在高 Se 溶液中，狐尾藻和金鱼藻中的含量分别为 48%-36%。使用 HPLC-ICP-MS 确定 Se 物种。两种植物中主要的可溶性物质均为硒酸盐（约 37%），而痕量水平检测到硒代蛋氨酸和硒代蛋氨酸亚砜。
