Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Burns. 2011 Nov;37(7):1202-7. doi: 10.1016/j.burns.2011.05.012. Epub 2011 Jun 24.
Extended spectrum β-lactamase (ESBL)-producing trait was present in 48 out of the 112 (42.8%) Pseudomonas aeruginosa isolates collected from burn wound infections during a 12-month period. The presence of oxa-10, per-1, veb-1 and ges genes and the multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) of 112 P. aeruginosa strains were determined by PCR and multiplex PCR. Disk diffusion methods were used to determine the susceptibility of the isolates to antimicrobial agents as instructed by CLSI. All ESBL isolates were resistant to aztreonam, cefepime, cefotaxime, cefpodoxime, ceftazidime, ceftriaxone and ofloxacin. Fewer than 60% of ESBL isolates were resistant to imipenem, meropenem, and piperacillin-tazobactam but more than 90% were resistant to amikacin, ciprofloxacin, levofloxacin, ticarcillin and tobramycin. The most prevalent ESBL genes included oxa-10 (70%) and per-1 (50%) followed by veb-1 (31.3%). The gene encodes GES enzyme did not detect in any isolates. A total of 100 P. aeruginosa strains were typed by MLVF typing method. MLVF produced 42 different DNA banding patterns. These data indicate that different MLVF types infect burn wounds in patients at a hospital in Tehran and also suggest an alarming rate of ESBL-producing isolates in this test location.
在 12 个月期间收集的 112 株来自烧伤感染的铜绿假单胞菌分离株中，有 48 株（42.8%）存在扩展谱β-内酰胺酶（ESBL）产生特性。通过 PCR 和多重 PCR 确定了 112 株铜绿假单胞菌菌株的 oxa-10、per-1、veb-1 和 ges 基因的存在以及多位点可变数串联重复（VNTR）指纹图谱（MLVF）。根据 CLSI 的指示，使用圆盘扩散法确定分离物对抗菌药物的敏感性。所有 ESBL 分离株均对氨曲南、头孢吡肟、头孢噻肟、头孢泊肟、头孢他啶、头孢曲松和氧氟沙星耐药。少于 60%的 ESBL 分离株对亚胺培南、美罗培南和哌拉西林他唑巴坦耐药，但超过 90%的分离株对阿米卡星、环丙沙星、左氧氟沙星、替卡西林和妥布霉素耐药。最常见的 ESBL 基因包括 oxa-10（70%）和 per-1（50%），其次是 veb-1（31.3%）。基因编码 GES 酶在任何分离株中均未检测到。总共对 100 株铜绿假单胞菌进行了 MLVF 分型。MLVF 产生了 42 种不同的 DNA 带型。这些数据表明，不同的 MLVF 类型感染了德黑兰一家医院的烧伤患者的伤口，并且在该测试地点也存在令人震惊的 ESBL 产生分离株率。
