National Synchrotron Radiation Laboratory, University of Science and Technology of China, and The First Affiliated Hospital, Hefei, Anhui 230029, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Sep;79(5):1660-2. doi: 10.1016/j.saa.2011.05.031. Epub 2011 Jun 7.
In order to investigate gastric cancer at cellular and sub-cellular level, a single human gastric adenocarcinoma BGC823 cell was studied by an infrared microscope equipped with a focal plane array (FPA) detector. The spectra showed difference between the nucleus and the endoplasmic reticulum (ER) of the BGC823 cell. The peak of vasPO2- was shifted to a higher wavenumber at the nucleus compared with that at the ER. The height ratios of 2954 cm(-1)/2922 cm(-1) (CH3/CH2) and 1088 cm(-1)/1539 cm(-1) (DNA/amide II) of the nucleus were significantly higher than those of the ER. Furthermore, chemical images reveal the intensity distributions of lipids, proteins and DNA of the single BGC823 cell, and the intense absorptions of proteins and DNA were observed in the nuclear region of the cell while the intense absorption of lipids was found in the ER region of the cell. The Fourier transform infrared (FTIR) microspectroscopic imaging result indicates the study of the single gastric cancer cell at sub-cellular level can be beneficial for knowing gastric cancer more which will be of great importance for the study and diagnosis of gastric cancer. The result also suggests that FPA is a useful tool in the study of a single cell and may be a powerful tool for study and diagnosis of gastric cancer.
为了在细胞和亚细胞水平上研究胃癌，我们使用配备有焦平面阵列（FPA）探测器的红外显微镜对单个人类胃腺癌 BGC823 细胞进行了研究。光谱显示 BGC823 细胞核与内质网（ER）之间存在差异。与 ER 相比，vasPO2-的峰在核中向更高的波数移动。核的 2954 cm(-1)/2922 cm(-1)（CH3/CH2）和 1088 cm(-1)/1539 cm(-1)（DNA/酰胺 II）峰的高度比明显高于 ER。此外，化学图像揭示了单个 BGC823 细胞的脂质、蛋白质和 DNA 的强度分布，在细胞的核区域观察到蛋白质和 DNA 的强烈吸收，而在 ER 区域则观察到脂质的强烈吸收。傅里叶变换红外（FTIR）微光谱成像结果表明，对亚细胞水平的单个胃癌细胞的研究有助于更好地了解胃癌，这对于胃癌的研究和诊断具有重要意义。该结果还表明，FPA 是研究单个细胞的有用工具，可能是研究和诊断胃癌的有力工具。
