Dresden University of Technology, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D, 01307 Dresden, Germany.
J Microbiol Methods. 2011 Sep;86(3):393-6. doi: 10.1016/j.mimet.2011.06.008. Epub 2011 Jun 17.
A PCR approach for multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae directly from respiratory samples was developed and evaluated on 54 specimens from M. pneumoniae-positive pneumonia patients. The method resulted in a clearly identifiable MLVA type in all samples tested and can be used for MLVA without laborious isolation of the pathogen.
建立了一种从呼吸道样本中直接检测肺炎支原体（Mycoplasma pneumoniae）多位点可变数串联重复分析（MLVA）的聚合酶链反应（PCR）方法，并对 54 例肺炎支原体阳性肺炎患者的标本进行了评估。该方法在所有检测样本中均产生了清晰可辨的 MLVA 型，无需费力分离病原体即可用于 MLVA。
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