Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario, Canada N5A 5B9.
Acta Biomater. 2011 Oct;7(10):3670-8. doi: 10.1016/j.actbio.2011.06.010. Epub 2011 Jun 13.
Efficient oxygen supply is a continuing challenge for the fabrication of successful tissue engineered constructs with clinical relevance. In an effort to enhance oxygen delivery we report the feasibility of using fluorinated zeolite particles embedded in three-dimensional (3-D) polyurethane scaffolds as novel oxygen vectors. First, 1H,1H,2H,2H-perfluorodecyltriethoxysilane was successfully coupled to zeolite framework particles to examine the dose-dependent dissolved oxygen concentration. Following this, the fluorinated-zeolite (FZ) particles were embedded in 3-D tissue engineering polyurethane scaffolds. Our data demonstrates an even distribution of FZ particles in the 3-D scaffolds without affecting the scaffold porosity or pore size. Human coronary artery smooth muscle cell (HCASMC) proliferation on FZ-containing polyurethane (PCU-FZ) scaffolds was significantly greater than on control scaffolds (P=0.05). Remarkably, cell infiltration depths on the PCU-FZ scaffolds was double that on PCU control scaffolds. Taken together, our data suggest the potential of PCU-FZ scaffolds for tissue engineering with enhanced oxygen delivery to cells.
高效供氧是成功制备具有临床相关性的组织工程构建体的持续挑战。为了提高氧的输送，我们报告了将嵌入三维（3-D）聚氨酯支架中的氟化沸石颗粒用作新型氧载体的可行性。首先，成功地将 1H，1H，2H，2H-全氟癸基三乙氧基硅烷偶联到沸石骨架颗粒上，以检查溶解氧浓度的剂量依赖性。在此之后，将氟化沸石（FZ）颗粒嵌入 3-D 组织工程聚氨酯支架中。我们的数据表明，FZ 颗粒在 3-D 支架中均匀分布，而不会影响支架的孔隙率或孔径。含 FZ 的聚氨酯（PCU-FZ）支架上的人冠状动脉平滑肌细胞（HCASMC）增殖明显大于对照支架（P=0.05）。值得注意的是，PCU-FZ 支架上细胞的渗透深度是 PCU 对照支架的两倍。总之，我们的数据表明，PCU-FZ 支架具有增强细胞氧输送的组织工程潜力。
