Yiangou Y, Serrano R, Bloom S R, Peña J, Festenstein H
Departamento de Bioquimica, Hospital Reina Sofia, Facultad de Medicina, Universidad de Cordoba, Spain.
J Neuroimmunol. 1990 Sep-Oct;29(1-3):65-72. doi: 10.1016/0165-5728(90)90148-g.
We have studied the effects of prepro-vasoactive intestinal polypeptide-derived peptides on lectin-induced lymphocyte proliferation and natural killer cell (NK) activity in cells from murine spleen, mesenteric lymph nodes, Peyer's patches, thymus and peripheral blood mononuclear lymphocytes (PBL). These peptides (vasoactive intestinal peptide (VIP), peptide histidine methionine (PHM-27) and peptide histidine valine (PHV-42)) showed differential effects in their immune response in a dose- and tissue-dependent manner. All peptides significantly decreased DNA synthesis in spleen (range: 45-30%), lymph nodes (range: 30-0%), Peyer's patches (range: 30-4%) and PBL (range: 30-16%). In these tissues there was no significant difference in their potency. In the thymus, however, PHM-27 (range: 27-15%) was significantly more potent (p less than 0.001) in inhibiting DNA synthesis than either VIP (range: 6-0%) or PHV-42 (range: 20-8%). The modulatory effects on NK activity by these peptides also showed an inhibitory effect. The order of potency was: VIP (range: 40-27%), PHV-42 (range: 22-11%) and PHM-27 (range: 20-8%). The presence of VIP inhibitor [( D-p-chloro-Phe6,Leu17]-VIP) at 10(-8) M in both functional assays caused a significant antagonism of the effects of VIP but not PHM-27 or PHV-42. Our results suggest the existence on lymphocytes of different receptors for prepro-VIP-derived peptides, and that they may be considered as important immunoregulatory molecules. Their mechanism of interaction, however, is not clearly understood.
我们研究了血管活性肠肽原衍生肽对小鼠脾脏、肠系膜淋巴结、派伊尔结、胸腺和外周血单个核细胞（PBL）中凝集素诱导的淋巴细胞增殖及自然杀伤细胞（NK）活性的影响。这些肽（血管活性肠肽（VIP）、肽组氨酸蛋氨酸（PHM - 27）和肽组氨酸缬氨酸（PHV - 42））在免疫反应中呈现出剂量和组织依赖性的差异效应。所有肽均显著降低了脾脏（范围：45% - 30%）、淋巴结（范围：30% - 0%）、派伊尔结（范围：30% - 4%）和PBL（范围：30% - 16%）中的DNA合成。在这些组织中，它们的效力无显著差异。然而，在胸腺中，PHM - 27（范围：27% - 15%）在抑制DNA合成方面比VIP（范围：6% - 0%）或PHV - 42（范围：20% - 8%）显著更有效（p小于0.001）。这些肽对NK活性的调节作用也呈现出抑制效应。效力顺序为：VIP（范围：40% - 27%）、PHV - 42（范围：22% - 11%）和PHM - 27（范围：20% - 8%）。在两种功能测定中，10(-8) M的VIP抑制剂[（D - p - 氯苯丙氨酸6，亮氨酸17）-VIP]对VIP的效应产生了显著拮抗作用，但对PHM - 27或PHV - 42无此作用。我们的结果表明淋巴细胞上存在血管活性肠肽原衍生肽的不同受体，并且它们可能被视为重要的免疫调节分子。然而，它们的相互作用机制尚不清楚。
