Department of Pharmaceutical Science, School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705, USA.
Nanomedicine. 2012 Feb;8(2):228-36. doi: 10.1016/j.nano.2011.06.009. Epub 2011 Jun 24.
Noninvasive near-infrared (NIR) fluorescence imaging is a promising technique for the intraoperative assessment of solid tumor removal. We incorporated a lipophilic NIR probe, 1,1'-dioctadecyltetramethyl indotricarbocyanine iodide (DiR), in poly(ethylene glycol)-b-poly(ɛ-caprolactone) (PEG-b-PCL) micelles, resulting in DiR solubilization in water, occupying nanoscopic PEG-b-PCL micelles. DiR in a self-quenched or nonquenched state showed different kinetics of release from PEG-b-PCL micelles in vitro; however, both obtained high tumor delineation (tumor-to-muscle ratio of 30-43 from collected organs). These results suggest that PEG-b-PCL micelles with DiR are a promising nanosized imaging agent that will provide a basis for enhanced surgical guidance via NIR visualization of tumors.
In this paper, noninvasive near-infrared fluorescence imaging coupled with specific lipophilic probes is discussed as a promising technique for intraoperative assessment of solid tumor removal, leading to optimized outcomes for in toto removal of tumors.
近红外（NIR）荧光非侵入性成像技术是一种很有前途的技术，可用于评估实体瘤切除的术中情况。我们将亲脂性近红外探针 1,1'-二辛基四甲基吲哚三碳氰（DiR）整合到聚乙二醇-聚（ε-己内酯）（PEG-b-PCL）胶束中，使 DiR 在水中溶解，占据纳米级 PEG-b-PCL 胶束。DiR 在自猝灭或非猝灭状态下，体外从 PEG-b-PCL 胶束中释放的动力学不同；然而，两者都获得了高肿瘤描绘（从采集的器官获得的肿瘤与肌肉比为 30-43）。这些结果表明，带 DiR 的 PEG-b-PCL 胶束是一种很有前途的纳米成像剂，将为通过 NIR 可视化肿瘤提供增强手术指导的基础。
本文讨论了将近红外荧光成像与特定亲脂性探针相结合作为一种很有前途的技术，用于评估实体瘤切除的术中情况，从而优化肿瘤全部切除的效果。
