Department of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India.
Int Immunopharmacol. 2011 Nov;11(11):1680-4. doi: 10.1016/j.intimp.2011.05.026. Epub 2011 Jun 24.
The present study was designed to evaluate the role of free radicals in restraint stress (RS)-induced modulation of immune responses in rats. RS significantly suppressed both humoral and cell-mediated immune responses as evidenced by reduced (a) anti-SRBC antibody titre (b) splenic Plaque Forming Cell counts, (c) footpad thickness response, and (d) IFN-γ and IL-4 levels. Assay for oxidative stress markers in blood showed that there was significant enhancement in plasma corticosterone and products of lipid peroxidation, viz. malondialdehyde and lowered reduced glutathione levels on exposure to RS. Further, this was associated with decreased antioxidant enzyme activity, viz. superoxide dismutase and catalase. These RS-induced changes in immunological and oxidative stress markers were markedly attenuated by pretreatment with the antioxidants, L-ascorbic acid (100 and 200 mg/kg) and α-tocopherol (30 and 60 mg/kg), by differential degrees. The combination of L-ascorbic acid and α-tocopherol was shown to have synergistic effects on reversal of these RS-induced effects. The results suggest that reactive oxygen species may be involved in stress-induced immunomodulation.
本研究旨在评估自由基在束缚应激(RS)诱导的大鼠免疫反应调节中的作用。RS 显著抑制了体液和细胞介导的免疫反应,表现为(a)抗绵羊红细胞抗体效价降低,(b)脾 Plaque 形成细胞计数降低,(c)足垫厚度反应降低,和(d)IFN-γ和 IL-4 水平降低。对血液中氧化应激标志物的检测表明,暴露于 RS 后,血浆皮质酮和脂质过氧化产物(如丙二醛)水平显著升高,而还原型谷胱甘肽水平降低。此外,这与抗氧化酶活性(如超氧化物歧化酶和过氧化氢酶)降低有关。用抗氧化剂 L-抗坏血酸(100 和 200mg/kg)和 α-生育酚(30 和 60mg/kg)预处理可显著减轻这些 RS 引起的免疫和氧化应激标志物的变化,程度不同。L-抗坏血酸和 α-生育酚的联合使用显示出对逆转这些 RS 诱导的作用具有协同效应。结果表明,活性氧可能参与应激诱导的免疫调节。