Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.
Anal Chim Acta. 2011 Aug 5;699(1):81-6. doi: 10.1016/j.aca.2011.05.012. Epub 2011 May 14.
A simple technique for quantitative analysis of four urinary biomarkers, tryptophan (TRP), 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) of carcinoid tumors is developed using gold nanoparticles as the assisted matrix in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS). The optimal SALDI conditions for the efficient ionization of those biomarkers are systematically explored by the adjustments of the concentrations of gold nanoparticles and internal standards. The mass spectra with strong signals and minimal background noise are obtained using 1-naphthaleneacetamide (NAD) as the internal standard. The calibration curves of the biomarker concentrations are determined using SALDI-TOF MS and the high linearity is obtained in all samples. For future clinical testing, multiplexed detection of those biomarkers in the urine samples of healthy males is performed. The successful quantitative detections of TRP, 5-HTP, 5-HT and 5-HIAA indicate that our technique provided great potentials to be developed a simple and rapid platform for the tumor biomarker detections.
一种简单的技术，用于定量分析 4 种尿生物标志物，色氨酸（TRP）、5-羟色氨酸（5-HTP）、5-羟色胺（5-HT）和 5-羟吲哚乙酸（5-HIAA）的类癌肿瘤，使用金纳米粒子作为辅助基质在表面辅助激光解吸/电离飞行时间质谱（SALDI-TOF MS）中。通过调整金纳米粒子和内标物的浓度，系统地探索了最佳的 SALDI 条件，以实现这些生物标志物的有效电离。使用 1-萘乙酰胺（NAD）作为内标物，获得了具有强信号和最小背景噪声的质谱图。使用 SALDI-TOF MS 确定了生物标志物浓度的校准曲线，并且在所有样品中都获得了高线性度。为了未来的临床测试，在健康男性的尿液样本中进行了这些生物标志物的多重检测。TRP、5-HTP、5-HT 和 5-HIAA 的成功定量检测表明，我们的技术具有很大的潜力，可以开发一种简单、快速的肿瘤生物标志物检测平台。
