Immunoassay for visualization of protein-protein interactions on Ni-nitrilotriacetate support: Example of a laboratory exercise with recombinant heterotrimeric Gα(i2) β(1γ2) Tagged by Hexahistidine from sf9 Cells.

We have developed an in vitro assay for following the interaction between the α(i2) subunit and β(1γ2) dimer from sf9 cells. This method is suitable for education purposes because it is easy, reliable, nonexpensive, can be applied for a big class of 20 students, and avoid the commonly used kinetic approach, which does not allow visualization of protein-protein interaction.