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口蹄疫病毒RNA 5'非编码区的一个区域指导细胞内蛋白质合成的有效内部起始：与L蛋白酶在翻译控制中的作用有关。

A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control.

作者信息

Belsham G J, Brangwyn J K

机构信息

AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.

出版信息

J Virol. 1990 Nov;64(11):5389-95. doi: 10.1128/JVI.64.11.5389-5395.1990.

DOI:10.1128/JVI.64.11.5389-5395.1990
PMID:2170677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248589/
Abstract

Plasmids encoding bicistronic mRNAs have been constructed and used to identify a region from the 5' noncoding region of foot-and-mouth disease virus (FMDV) which directs efficient internal initiation of protein synthesis within cells. The loss of about 30 nucleotides (nt) from the 5' terminus or about 50 nt from the 3' terminus of the 435-nt region completely abolished the activity of this region. The expression of the FMDV L protease severely inhibited the expression of other genes unless they were preceded by this element. The regulation of protein synthesis mediated by FMDV is discussed.

摘要

已经构建了编码双顺反子mRNA的质粒，并用于鉴定口蹄疫病毒（FMDV）5'非编码区中的一个区域，该区域可指导细胞内蛋白质合成的有效内部起始。从435个核苷酸（nt）区域的5'末端缺失约30个核苷酸（nt）或从3'末端缺失约50个nt会完全消除该区域的活性。除非其他基因之前有该元件，否则FMDV L蛋白酶的表达会严重抑制其他基因的表达。本文讨论了由FMDV介导的蛋白质合成调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a7/248589/2ce4b8eb86be/jvirol00066-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a7/248589/63cf429ce65f/jvirol00066-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a7/248589/2ce4b8eb86be/jvirol00066-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a7/248589/63cf429ce65f/jvirol00066-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4a7/248589/2ce4b8eb86be/jvirol00066-0170-a.jpg

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本文引用的文献

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