雌激素增强了ZR-75-1人乳腺癌细胞中MMTV-LTR对糖皮质激素的反应性。

Oestrogen enhances the responsiveness of the MMTV-LTR to glucocorticoid in ZR-75-1 human breast cancer cells.

作者信息

Bansal G S, Latchman D S

机构信息

Department of Biochemistry, University College and Middlesex School of Medicine, London, England.

出版信息

J Steroid Biochem. 1990 Aug 14;36(5):399-405. doi: 10.1016/0022-4731(90)90080-c.

DOI:10.1016/0022-4731(90)90080-c

PMID:2170763

Abstract

Treatment of ZR-75-1 human breast cancer cells with oestrogen has no direct effect on the expression of a transfected MMTV-LTR but enhances its inducibility in response to glucocorticoid treatment. This effect which can be produced with both oestradiol and diethylstilbestrol is specific to induction of the MMTV-LTR, no effect of the treatment on expression driven by the RSV-LTR being observed. The effect can be observed in cells pre-treated with oestrogen prior to removal of DNA and glucocorticoid addition but not in cells where oestrogen is added after removal of the DNA. The possible mechanisms of these effects and their relationship to the induction of oestrogen-responsive genes by this hormone are discussed.

摘要

用雌激素处理ZR-75-1人乳腺癌细胞对转染的MMTV-LTR的表达没有直接影响，但会增强其对糖皮质激素处理的诱导性。雌二醇和己烯雌酚均可产生这种效应，它对MMTV-LTR的诱导具有特异性，未观察到该处理对由RSV-LTR驱动的表达有影响。在去除DNA并添加糖皮质激素之前先用雌激素预处理的细胞中可观察到这种效应，但在去除DNA后添加雌激素的细胞中则未观察到。本文讨论了这些效应的可能机制及其与该激素诱导雌激素反应性基因的关系。