State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Plant Cell Environ. 2011 Sep;34(9):1586-98. doi: 10.1111/j.1365-3040.2011.02356.x. Epub 2011 Jun 28.
The molecular mechanisms of signal transduction of plants in response to infection by Verticillium dahliae (VD) are not well understood. We previously showed that NO may act as an upstream signalling molecule to trigger the depolymerization of cortical microtubules in Arabidopsis. In the present study, we used the wild-type, and atrbohD and atrbohF mutants of Arabidopsis to explore the mechanisms of action of H(2)O(2) signals and the dynamic microtubule cytoskeleton in defence responses. We demonstrated that H(2)O(2) may also act as an upstream signalling molecule to regulate cortical microtubule depolymerization. The depolymerization of the cortical microtubules played a functional role in the signalling pathway to mediate the expression of defence genes. The results indicate that H(2)O(2) modulates the dynamic microtubule cytoskeleton to trigger the expression of defence genes against V. dahliae toxins (VD-toxins) in Arabidopsis.
植物对黄萎病菌（VD）感染的信号转导的分子机制尚不清楚。我们之前的研究表明，NO 可能作为一种上游信号分子，触发拟南芥皮层微管的解聚。在本研究中，我们使用野生型、atrbohD 和 atrbohF 拟南芥突变体，来探讨 H2O2 信号和动态微管细胞骨架在防御反应中的作用机制。我们证明 H2O2 也可能作为一种上游信号分子，调节皮层微管的解聚。皮层微管的解聚在信号通路中发挥功能作用，介导防御基因的表达。结果表明，H2O2 调节动态微管细胞骨架，以触发拟南芥对黄萎病菌毒素（VD-toxins）的防御基因的表达。
