Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Lett Appl Microbiol. 2011 Sep;53(3):313-23. doi: 10.1111/j.1472-765X.2011.03108.x. Epub 2011 Jul 25.
To study the genetic relatedness between V. cholerae isolates from Iran and other countries based on housekeeping gene recA sequence analysis.
A 995-bp region of the recA gene from 24 V. cholerae isolates obtained from human and surface water origins in Iran over a 5-year period was sequenced and compared with the sequence data from the isolates belonging to other places. Cluster analysis of the constructed dendrogram based on recA sequence divergence for our clinical isolates showed one sequence type (ST), whereas environmental isolates revealed eight STs. Interestingly, one of our environmental isolates was intermixed with clinical isolates in the largest cluster containing the epidemic strains. Our 24 isolates plus 198 global isolates available in the GenBank showed 77 sequence types (STs) with at least one nucleotide difference.
Our result suggested that recA sequencing is a reliable analysis method for understanding the relatedness of the local isolates with the isolates obtained elsewhere.
Understanding the genetic relatedness between V. cholerae isolates could give insights into the health care system for better control and prevention of the cholera.
基于管家基因 recA 序列分析，研究伊朗和其他国家分离的霍乱弧菌之间的遗传相关性。
对伊朗 5 年内从人和地表水来源获得的 24 株霍乱弧菌分离株的 recA 基因的 995bp 区域进行测序，并与属于其他地方的分离株的序列数据进行比较。根据 recA 序列差异构建的聚类分析树状图显示，我们的临床分离株只有一个序列型（ST），而环境分离株则有 8 个 ST。有趣的是，我们的一个环境分离株与最大簇中包含流行株的临床分离株混合在一起。我们的 24 株分离株加上 GenBank 中可用的 198 株全球分离株显示至少有一个核苷酸差异的 77 个序列型（ST）。
我们的结果表明，recA 测序是一种可靠的分析方法，可用于了解本地分离株与其他地方获得的分离株之间的相关性。
了解霍乱弧菌分离株之间的遗传相关性可以深入了解医疗保健系统，从而更好地控制和预防霍乱。
