Farhadi Tayebeh, Nezafat Navid, Ghasemi Younes
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran; Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
Int J Comput Biol Drug Des. 2015;8(1):62-74. doi: 10.1504/IJCBDD.2015.068789. Epub 2015 Apr 13.
Vibrio cholera, a gram-negative bacterium, has been categorised into clinical and environmental species. Phylogenetic studies have been performed to investigate the relationships of the V. cholerae populations in worldwide. In this study, phylogenetic relationship between V. cholerae isolates from Iran and other regions of the world was determined, based on three housekeeping genes analysis. Results for Iranian strains showed that congruency of asd and hlyA phylogenetic trees were remarkably higher than recA tree. Iranian strains displayed 2-3%, 1-14% and 3-5% deference in asd, hlyA and recA nucleotide sequences, respectively. Sequence similarity degrees were variable between Iranian and other region's strains. Furthermore, the non-congruence in the phylogeny of the pathogenic clones in cladograms is probably due to horizontal gene transfer. Finally, results of this study suggest that monitoring of surface waters for housekeeping genes of V. cholerae in the cholera endemic areas may be valuable for forecasting the expected cholera outbreaks.
霍乱弧菌是一种革兰氏阴性菌，已被分为临床型和环境型。人们已经开展了系统发育研究，以调查全球范围内霍乱弧菌种群之间的关系。在本研究中，基于对三个管家基因的分析，确定了来自伊朗的霍乱弧菌分离株与世界其他地区霍乱弧菌之间的系统发育关系。伊朗菌株的结果表明，天冬氨酸半醛脱氢酶（asd）和溶血素A（hlyA）系统发育树的一致性明显高于重组酶A（recA）树。伊朗菌株在asd、hlyA和recA核苷酸序列上分别显示出2 - 3%、1 - 14%和3 - 5%的差异。伊朗菌株与其他地区菌株之间的序列相似程度各不相同。此外，进化枝图中致病克隆系统发育的不一致可能是由于水平基因转移造成的。最后，本研究结果表明，在霍乱流行地区监测地表水以检测霍乱弧菌的管家基因，可能对预测预期的霍乱疫情很有价值。
