Ferrari S, Ceccherelli G, Tagliafico E, Zucchini P, Manfredini R, Torelli G, Emilia G, Torelli U
Second Medical Clinic, University of Modena, Italy.
Leukemia. 1990 Oct;4(10):688-93.
The hybridization to a complementary RNA (cRNA) probe both in situ and in solution was used to assay tiny amounts of mRNA of the lactoferrin (LF) and myeloperoxidase (MPO) genes in normal bone marrow cells and in acute and chronic lymphoid leukemias. Evidence is reported that this technique is much more sensitive than the standard Northern blot technique. The LF mRNA was detectable in three of seven cases of acute lymphoblastic leukemia (ALL) and in three of seven cases of chronic lymphocytic leukemia (CLL). Four cases of ALL were also positive when tested with the MPO cRNA. It is apparent from these results that myeloid specific mRNA, different from MPO, may be detected in leukemic cells with lymphoid phenotype using a method more sensitive than the Northern blot technique. Whether or not the molecular events observed in these cell populations reflect events physiologically occurring rather than a deregulation of gene expression associated to leukemogenesis remains to be established.
采用原位和溶液中与互补RNA（cRNA）探针杂交的方法，检测正常骨髓细胞以及急慢性淋巴细胞白血病中乳铁蛋白（LF）和髓过氧化物酶（MPO）基因的微量mRNA。有证据表明，该技术比标准的Northern印迹技术更为灵敏。在7例急性淋巴细胞白血病（ALL）中有3例可检测到LF mRNA，7例慢性淋巴细胞白血病（CLL）中有3例可检测到LF mRNA。用MPO cRNA检测时，4例ALL也呈阳性。从这些结果可以明显看出，使用比Northern印迹技术更灵敏的方法，在具有淋巴样表型的白血病细胞中可以检测到不同于MPO的髓系特异性mRNA。这些细胞群体中观察到的分子事件是否反映生理发生的事件，而不是与白血病发生相关的基因表达失调，仍有待确定。
