Research Center, Iranian Blood Transfusion Organization, Tehran, Iran.
Int J Lab Hematol. 2011 Dec;33(6):586-92. doi: 10.1111/j.1751-553X.2011.01354.x. Epub 2011 Jun 27.
During recent years, the need for platelet concentrate (PC) has increased. Infusible platelet membranes (IPM) have been developed as an alternative to standard PCs, with the additional advantage of long shelf-life and increased viral safety. In this study, IPM construction and the morphological and biological features of these microvesicles were surveyed to determine their binding capacities in vitro.
Thirty-five PC units prepared by the Iranian Blood Transfusion Organization were used to produce IPM. Platelets were lysed by repeated cycles of freezing and thawing, virally inactivated with wet heat in the presence of different concentrations of sodium octanoate as a heat stabilizer, and then sonicated. IPM were separated, kept at 4°C or lyophilized, and examined for binding to collagen and von Willebrand factor (VWF).
IPM retained the binding capacity for collagen and VWF, and the extent of VWF binding was dependent on the concentration of the heat stabilizer. Additionally, a higher binding capacity was demonstrated for liquid-stored compared with lyophilized IPM.
This study revealed the potential of IPM microvesicles to mimic the binding features of platelets in vitro.
近年来，对血小板浓缩物（PC）的需求不断增加。不可输注的血小板膜（IPM）已被开发为标准 PCs 的替代品，具有更长的货架寿命和更高的病毒安全性的额外优势。在这项研究中，我们调查了 IPM 的构建以及这些微囊泡的形态和生物学特征，以确定它们在体外的结合能力。
使用 35 个由伊朗输血组织制备的 PC 单位来生产 IPM。血小板通过反复冷冻和解冻循环裂解，在存在不同浓度的辛酸钠作为热稳定剂的情况下用湿热法失活，然后进行超声处理。IPM 被分离出来，在 4°C 下保存或冻干，并检查它们与胶原蛋白和血管性血友病因子（VWF）的结合能力。
IPM 保留了与胶原蛋白和 VWF 的结合能力，VWF 的结合程度取决于热稳定剂的浓度。此外，与冻干的 IPM 相比，液态储存的 IPM 表现出更高的结合能力。
这项研究揭示了 IPM 微囊泡在体外模拟血小板结合特征的潜力。
