Abdanipour Alireza, Tiraihi Taki, Mirnajafi-Zadeh Javad
Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Neurol Res. 2011 Jul;33(6):625-32. doi: 10.1179/1743132810Y.0000000018.
Temporal lobe epilepsy is one of the most common types of epilepsy. Cytotherapy was tried for several neurological disorders. In this investigation, bone marrow stromal cells (BMSCs) were used in order to evaluate the recovery of epileptic rats induced by pilocarpine.
The rats were divided into four groups: a negative control, treated groups, and epileptic rats treated with the vehicle only. The animals in the chronic phase were monitored for three weeks using Racine scale. BMSCs were labeled with BrdU and injected intravenously. After 6 weeks, the rats were killed and processed in paraffin as well as immunohistochemical techniques.
The results of the behavioral test showed that the number of seizures significantly decreased in treatment groups. Histopathology of the tissues from the untreated rats showed cell death and neurophagia. The numerical density of neurons per area was significantly higher in the treated groups than in the untreated ones. BMSCs localized in the hippocampus of the treated animals.
The results showed that there is structural and functional improvement in the epileptic rats treated with BMSCs.
颞叶癫痫是最常见的癫痫类型之一。细胞疗法已用于多种神经系统疾病的治疗。在本研究中，为评估匹鲁卡品诱导的癫痫大鼠的恢复情况，使用了骨髓基质细胞（BMSCs）。
将大鼠分为四组：阴性对照组、治疗组以及仅用赋形剂处理的癫痫大鼠组。使用拉辛量表对慢性期动物进行为期三周的监测。用溴脱氧尿苷（BrdU）标记BMSCs并静脉注射。6周后，处死大鼠并进行石蜡包埋及免疫组织化学技术处理。
行为测试结果显示，治疗组癫痫发作次数显著减少。未治疗大鼠组织的组织病理学检查显示细胞死亡和噬神经细胞现象。治疗组每单位面积神经元的数量密度显著高于未治疗组。BMSCs定位于治疗动物的海马体中。
结果表明，用BMSCs治疗的癫痫大鼠在结构和功能上均有改善。
