Fujii Y, Sendo F, Kamiyama T, Naiki M
Institute for Laboratory Animal Research, Nagoya University School of Medicine.
Microbiol Immunol. 1990;34(6):533-42.
IgG and IgM antibodies, which were isolated from the anti-asialoGM1 (GA1) serum, had different effects against natural killer (NK) and prematured cytotoxic T cells (CTLs) in in vivo administration and in in vitro treatment. In in vitro treatments, the IgM antibody killed NK cells of nude mouse spleen in the presence of complement (C') 12 times more potently than the IgG antibody did, and either antibody with C' killed pre-CTL. In in vivo administrations, only the IgG antibody was effective in diminishing NK activity of the nude mouse spleen cells and in suppressing antigen-specific CTL induction from primed spleen cells by in vitro stimulation with X-irradiated tumor cells. The IgM antibody was not effective at all in either system. The in vivo effect of the IgG on NK activity was blocked by preadministration with silica or carrageenan but not by that with cobra venom factor (CVF). These results indicate that in vivo administration of anti-GA1 antiserum leads to macrophage-mediated depletion of CTL precursors as well as NK cells.
从抗去唾液酸GM1（GA1）血清中分离出的IgG和IgM抗体，在体内给药和体外处理中，对自然杀伤（NK）细胞和早熟细胞毒性T细胞（CTL）有不同影响。在体外处理中，IgM抗体在补体（C'）存在的情况下杀死裸鼠脾脏NK细胞的效力比IgG抗体高12倍，并且两种抗体与C'一起都能杀死前CTL。在体内给药中，只有IgG抗体在降低裸鼠脾脏细胞的NK活性以及通过用X射线照射的肿瘤细胞进行体外刺激来抑制致敏脾细胞的抗原特异性CTL诱导方面有效。IgM抗体在这两种系统中均完全无效。IgG对NK活性的体内作用可被预先给予二氧化硅或角叉菜胶阻断，但不能被眼镜蛇毒因子（CVF）阻断。这些结果表明，体内给予抗GA1抗血清会导致巨噬细胞介导的CTL前体以及NK细胞的耗竭。
