Zadesenets Kira S, Katokhin Alexei V, Mordvinov Viatcheslav A, Rubtsov Nikolay B
Institute of Cytology and Genetics Siberian Branch of RAS, Novosibirsk, Russia.
Parasitol Int. 2012 Mar;61(1):81-3. doi: 10.1016/j.parint.2011.06.013. Epub 2011 Jun 25.
The analysis of telomere repeat distribution in chromosomes of five opisthorchid species (Opisthorchis felineus (Rivolta, 1884), Opisthorchis viverrini (Poirier, 1886), Metorchis xanthosomus (Creplin, 1846), Metorchis bilis (Braun, 1890), Clonorchis sinensis (Cobbold, 1875)) was performed with fluorescent in situ hybridization (FISH) of labeled (TTAGGG)n DNA-probe and PNA telomere probe on mitotic and meiotic chromosomes of these species. It was shown that chromosome telomeres of all studied species contain large clusters of (TTAGGG)n telomeric repeats. Interstitial clusters of the (TTAGGG)n repeats have not been revealed in the chromosomes of any studied species even when FISH of PNA telomere probe on pachytene chromosomes was performed. Furthermore interstitial clusters of the (TTAGGG)n repeats have not been detected in the chromosomes of O. viverrini, one of chromosomes of this species is the result of a fusion of two ancestral opisthorchid chromosomes.
利用标记的（TTAGGG）n DNA探针和PNA端粒探针，对5种后睾吸虫（猫后睾吸虫（Rivolta，1884年）、麝猫后睾吸虫（Poirier，1886年）、黄后睾吸虫（Creplin，1846年）、胆后睾吸虫（Braun，1890年）、华支睾吸虫（Cobbold，1875年））染色体中的端粒重复序列分布进行了有丝分裂和减数分裂染色体荧光原位杂交（FISH）分析。结果表明，所有研究物种的染色体端粒均含有大量（TTAGGG）n端粒重复序列簇。即使对粗线期染色体进行PNA端粒探针FISH，在任何研究物种的染色体中均未发现（TTAGGG）n重复序列的间质簇。此外，在麝猫后睾吸虫的染色体中未检测到（TTAGGG）n重复序列的间质簇，该物种的一条染色体是两条祖先后睾吸虫染色体融合的结果。
