Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
J Chromatogr A. 2011 Aug 5;1218(31):5248-53. doi: 10.1016/j.chroma.2011.06.005. Epub 2011 Jun 12.
A simple analytical method was developed for the simultaneous analysis of bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) in plant oil. The target compounds were extracted by cyclohexane/ethyl acetate (1:1), purified by gel permeation chromatography (GPC), and analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the negative ion mode. An isolator column was attached in front of the injection valve of the LC to separate background contaminants. Recovery studies were performed at three fortification levels. Mean recoveries were from 92.9% to 119.0%, with an acceptable coefficient of variation (4.4-18.5%, n=6). The limits of quantification of the method were 2, 2 and 0.5 μg/kg for BPA, NP and OP, respectively. This method can be applied for screening and confirming target compounds in plant oil.
建立了一种同时分析植物油中双酚 A（BPA）、壬基酚（NP）和辛基酚（OP）的简单分析方法。目标化合物用环己烷/乙酸乙酯（1:1）提取，经凝胶渗透色谱（GPC）纯化，在负离子模式下用液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）分析。在 LC 的进样阀前连接一个隔离柱，以分离背景污染物。在三个加标水平进行了回收率研究。平均回收率为 92.9%至 119.0%，变异系数可接受（4.4-18.5%，n=6）。该方法对 BPA、NP 和 OP 的定量限分别为 2、2 和 0.5 μg/kg。该方法可用于筛选和确证植物油中的目标化合物。
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