Am J Bot. 1997 Dec;84(12):1658.
Although microsatellites or simple sequence repeats (SSRs) have become a popular tool in genetic mapping and gene flow studies, their utility is limited due to paucity of information about DNA sequences in plants. We tested the utility of microsatellite markers characterized for the tropical tree Pithecellobium elegans as a genetic tool for related species. The results indicate that SSR loci are conserved among closely related species, and SSR primers developed for P. elegans could be successfully used as a genetic tool in several species of the tribe Ingeae. This study indicates that there is high potential for the transfer of SSR markers among closely related taxa, circumventing laborious cloning and screening procedures involved in characterizing SSR loci for many species.
尽管微卫星或简单重复序列 (SSR) 已成为遗传图谱和基因流研究中一种流行的工具,但由于植物中有关 DNA 序列的信息匮乏,它们的用途受到限制。我们测试了为热带树木 Pithecellobium elegans 所特有的微卫星标记作为相关物种遗传工具的实用性。结果表明,SSR 位点在近缘物种中是保守的,并且为 P. elegans 开发的 SSR 引物可以成功地作为 Ingeae 族的几个物种的遗传工具。这项研究表明,在近缘分类群之间转移 SSR 标记具有很大的潜力,可以避免为许多物种进行 SSR 位点的特征描述所涉及的繁琐克隆和筛选程序。