Naik Aparupa, Mishra Sujit K, Nag Atul, Soren Gopal K, Panda Aditya K, Panda Sanjib K, Panigrahi Jogeswar
Department of Bioscience and Bioinformatics, Khallikote University, Konishi, Berhampur, Odisha 761008 India.
Department of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019 India.
Physiol Mol Biol Plants. 2020 Dec;26(12):2371-2390. doi: 10.1007/s12298-020-00907-x. Epub 2020 Nov 21.
(L.) is a medicinal leguminous plant and is cultivated to cater the need of herbal industries and asthetic purposes. The unavailability of steady molecular marker impedes the genetic improvement of . In the present study, transferability of 98 pairs of specific SSR primers were assessed among 14 genotypes of , varied for their flower color, floral architecture and bio-metabolite (taraxerol and delphinidin) content, and out of them 43 had successfully amplified the fragments. Among them, 36 pairs of primers showed 100% transferability, whereas rest seven varied from 42.86 to 92.85% transferability. The transferable 43 pairs of SSR primers generated 196 alleles across the 14 genotypes and the AMOVA analysis showed moderate genetic variation (55.1%) among the genotypes of , which was also reinforced by Nei's genetic distance and gene identity estimates derived haplotype matrix. Similarly, both the principal coordinate analysis and dendrogram grouped these 14 genotypes of into two major clusters based on SSR allele distribution and frequency, and the clustering pattern is in accordance with petal color but in contrast to floral architecture. based outlier analysis revealed 16 alleles for balancing selection, which are putatively involved in the maintenance of genetic polymorphism in . Moreover, the estimates of molecular diversity and bio-metabolite content revealed the possible use of these genotypes in future breeding programme of this species.
（L.）是一种药用豆科植物，其种植是为了满足草药产业的需求和审美目的。稳定分子标记的缺乏阻碍了（该植物名称缺失）的遗传改良。在本研究中，评估了98对（该植物名称缺失）特异性SSR引物在14个基因型（该植物名称缺失）中的可转移性，这些基因型在花色、花结构和生物代谢物（蒲公英萜醇和飞燕草色素）含量方面存在差异，其中43对引物成功扩增出片段。其中，36对引物显示出100%的可转移性，而其余7对引物的可转移性在42.86%至92.85%之间变化。可转移的43对SSR引物在14个基因型中产生了196个等位基因，AMOVA分析显示（该植物名称缺失）基因型之间存在中等程度的遗传变异（55.1%），基于单倍型矩阵的Nei遗传距离和基因同一性估计也证实了这一点。同样，主坐标分析和聚类图都根据SSR等位基因分布和频率将这14个（该植物名称缺失）基因型分为两个主要聚类，聚类模式与花瓣颜色一致，但与花结构相反。基于（该植物名称缺失）的离群值分析揭示了16个用于平衡选择的等位基因，这些等位基因可能参与了（该植物名称缺失）遗传多态性的维持。此外，分子多样性和生物代谢物含量的估计揭示了这些基因型在该物种未来育种计划中的可能用途。
