Am J Bot. 1997 Apr;84(4):555.
Data from restriction-site variation of three PCR-amplified chloroplast genic regions (trnK, rps2, and rbcL) were used to assess the utility of PCR-based methodology for phylogenetic reconstruction. Seventeen genera from tribe Cheloneae s.l. (Scrophulariaceae), and one genus each from Solanaceae, Acanthaceae, and Bignoniaceae, representing 32 taxa, were sampled. Phylogenetic reconstruction, based on a combined data set of 138 variable restriction sites, revealed a monophyletic clade of North American Cheloneae, which were not inconsistent with a polyphyletic Scrophulariaceae. Separate analyses of individual genie regions were unable to completely resolve the phylogeny, but were adequate for resolving relationships of major clades among the taxa sampled. We suggest that analysis of PCR-product restriction-site variation is useful for phylogenetic reconstruction above the species level.
使用来自三个叶绿体基因区域（trnK、rps2 和 rbcL）的 PCR 扩增限制位点变化的数据来评估基于 PCR 的方法在系统发育重建中的效用。从玄参科 Cheloneae 族（玄参科）的 17 个属，茄科、爵床科和紫葳科的每个属各 1 个属，代表 32 个分类单元，进行了采样。基于 138 个可变限制位点的组合数据集的系统发育重建揭示了北美的 Cheloneae 是一个单系群，与多系的玄参科不一致。个别基因区域的单独分析无法完全解决系统发育问题，但足以解决所采样分类单元之间的主要分支关系。我们建议，分析 PCR 产物限制位点变化对于种以上水平的系统发育重建是有用的。
