Gaudry M, Marquetty C, Hakim J
Laboratoire d'Hématologie et d'Immunologie, INSERM U 294, CHU Xavier-Bichat, Paris, France.
Nouv Rev Fr Hematol (1978). 1990;32(3):191-8.
Phorbol myristate acetate (PMA) is a potent activator of Ca2+/phospholipid-dependent protein kinase (PKC) and was used to study the involvement of this kinase in human polymorphonuclear neutrophil (PMN) locomotion. Preincubation of PMNs with low concentrations of PMA (4 to 64 x 10(-11) M) had the following effects: (1) fMet-Leu-Phe-induced migration under agarose was increased when the chemoattractant was used at the suboptimal concentration of 10(-8)M and not at the optimal concentration of 10(-7)M; (2) no effect on spontaneous or serum- or LTB4- induced migration at either suboptimal or optimal concentrations; (3) PMA enhanced fMet-Leu-Phe-induced migration, increasing the speed of locomotion but not affecting shape changes induced by fMet-Leu-Phe; (4) the number of fMet-Leu-Phe-specific receptors expressed on the PMN membrane was not altered. Intermediate concentrations of PMA (1.6 to 4.0 x 10(-9) M) had no effect on PMN migration, whereas higher concentrations (4.0 to 16 x 10(-9) M) reduced both spontaneous and fMet-Leu-Phe-, serum-, or LTB4-induced migration in a dose-dependent manner. Effects observed with low concentrations of PMA were not associated with a translocation of cytosolic PKC even when preincubation with PMA was followed by fMet-Leu-Phe stimulation. In contrast, effects observed with higher concentrations of PMA paralleled the decrease in cytosolic PKC activity.
佛波醇肉豆蔻酸酯乙酸盐（PMA）是一种有效的Ca2+/磷脂依赖性蛋白激酶（PKC）激活剂，被用于研究该激酶在人多形核中性粒细胞（PMN）运动中的作用。用低浓度的PMA（4至64×10⁻¹¹M）对PMN进行预孵育产生了以下影响：（1）当趋化剂以10⁻⁸M的次优浓度而非10⁻⁷M的最佳浓度使用时，fMet-Leu-Phe诱导的琼脂糖下迁移增加；（2）在次优或最佳浓度下，对自发迁移或血清或LTB4诱导的迁移均无影响；（3）PMA增强了fMet-Leu-Phe诱导的迁移，提高了运动速度，但不影响fMet-Leu-Phe诱导的形态变化；（4）PMN膜上表达的fMet-Leu-Phe特异性受体数量未改变。中等浓度的PMA（1.6至4.0×10⁻⁹M）对PMN迁移无影响，而较高浓度（4.0至16×10⁻⁹M）则以剂量依赖性方式降低自发迁移以及fMet-Leu-Phe、血清或LTB4诱导的迁移。低浓度PMA所观察到的效应与胞质PKC的转位无关，即使在PMA预孵育后再用fMet-Leu-Phe刺激也是如此。相反，高浓度PMA所观察到的效应与胞质PKC活性的降低平行。
