Department of Physical Chemistry, Chalmers University of Technology, Kemivägen 10, SE-41296, Göteborg, Sweden.
Analyst. 2011 Aug 21;136(16):3303-6. doi: 10.1039/c0an00455c. Epub 2011 Jun 27.
This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost.
本文提出了一种新的流动取向装置设计，用于使用线二色性（LD）光谱研究生物大分子，包括 DNA 和蛋白质复合物，以及淀粉样纤维和脂质体膜等聚集体。该设计提供了许多技术优势，应该使该装置制造成本低廉、使用更方便、比现有技术更可靠。所达到的取向程度与常用的同心圆筒库埃特流池相当，但由于该装置利用了一组无应变的平板石英片，消除了与光折射和双折射相关的许多问题，提高了测量的灵敏度和准确性。该装置提供与库埃特池相似的剪切速率，但优于库埃特池的是，在整个间隙中剪切速率是恒定的。该设计的其他主要优点是可以轻松、低成本地更换部件和改变样品体积和路径长度。
