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通过复乳聚合合成的用于蛋白质分析的多种大孔球。

Diverse macroporous spheres synthesized by multiple emulsion polymerization for protein analyses.

机构信息

Department of Chemistry, National Cheng Kung University, No. 1 College Road, Tainan, Taiwan.

出版信息

Chem Commun (Camb). 2011 Aug 7;47(29):8385-7. doi: 10.1039/c1cc12167g. Epub 2011 Jun 27.

DOI:10.1039/c1cc12167g
PMID:21709876
Abstract

Hydrophilic and hydrophobic spheres (1-2 mm) with macropores (50 μm) were synthesized by SDS stabilized O/W/O and W/O/W emulsion polymerization, respectively. The hydrophilic spheres were functionalized with trypsin for protein digestion and on-line integrated with the hydrophobic spheres for the subsequent sample clean-up and enrichment with almost no back pressure.

摘要

亲水和疏水球(1-2 毫米)分别通过 SDS 稳定的 O/W/O 和 W/O/W 乳液聚合合成,具有大孔(50μm)。亲水球用胰蛋白酶进行蛋白质消化功能化,并与疏水球在线集成,用于随后的样品净化和富集,几乎没有背压。

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