Al-Ghamdi Ali, Morsy Kareem, Bashtar Abdel-Rahman, Abdel-Ghaffar Fathy, Al-Rasheid Khaled, Al-Quraishy Saleh, Mehlhorn Heinz
Zoology Department, College of Science, Al Baha University, Al Baha, Saudi Arabia.
J Parasitol. 2011 Oct;97(5):878-83. doi: 10.1645/GE-2778.1. Epub 2011 Jun 28.
The life cycle of Hepatozoon hemprichi n. sp. is described; the vertebrate host is Scincus hemprichii and it is vectored by Hyalomma impeltatum. Erythrocytic stages of 18 ± 1.8 × 4 ± 0.8 µm developed in the hemocoel of ticks to sporozoites within 16-18 days. Schizogony occurred in the liver parenchyma and the endothelial cells of blood capillaries in lung and spleen. Mature schizonts measuring 27 ± 3.11 × 20.13 ± 3.0 µm produced 28 merozoites (on average). The merozoites were 13 ± 1.21 × 1.21 ± 0.72 µm with nuclei 5 ± 0.65 × 2.1 ± 0.51 µm. Syzygy and differentiation of gamonts took place in tick's hemocoel up to the third day post-infection (PI). The microgamont (16 ± 0.31 × 18 ± 0.42 µm) produced 4, uniflagellated microgametes at 4-5 days PI. The microgamete measured 15.2 ± 0.31 µm while the flagellum was always at least 26 µm. The macrogamete was very large in size (31 ± 3.11 µm) with a central nucleus. After fertilization, (5-6 days PI) zygotes developed into oocysts (55 ± 3.41 × 52 ± 4.11 µm) in which repeated mitotic divisions with centripetal invaginations occurred; each contained 18 banana-shaped sporozoites, 13.61 ± 0.8 × 1.2 ± 0.31 µm in size. Experimental transmission was successfully carried out by oral administration or by intra-peritoneal inoculation of the infective stages (sporozoites) to uninfected skinks and led to the appearance of blood stages after 5 wk and 4 wk, respectively.
描述了新种亨氏肝簇虫（Hepatozoon hemprichi n. sp.）的生命周期；脊椎动物宿主为亨氏沙蜥（Scincus hemprichii），传播媒介为边缘璃眼蜱（Hyalomma impeltatum）。在蜱的血腔中，18 ± 1.8 × 4 ± 0.8 µm的红细胞内期在16 - 18天内发育为子孢子。裂体生殖发生在肝实质以及肺和脾的毛细血管内皮细胞中。成熟裂殖体大小为27 ± 3.11 × 20.13 ± 3.0 µm，平均产生28个裂殖子。裂殖子大小为"13 ± 1.21 × 1.21 ± 0.72 µm，细胞核大小为5 ± 0.65 × 2.1 ± 0.51 µm。配子母细胞的配对和分化在感染后第3天之前发生在蜱的血腔中。小配子母细胞（16 ± 0.31 × 18 ± 0.42 µm）在感染后4 - 5天产生4个单鞭毛小配子。小配子大小为15.2 ± 0.31 µm，鞭毛长度总是至少26 µm。大配子体积非常大（31 ± 3.11 µm），有一个位于中央的细胞核。受精后（感染后5 - 6天），合子发育为卵囊（55 ± 3.41 × 52 ± 4.11 µm），其中发生向心内陷的反复有丝分裂；每个卵囊含有18个香蕉形子孢子，大小为13.61 ± 0.8 × 1.2 ± 0.31 µm。通过向未感染的沙蜥口服或腹腔接种感染期（子孢子）成功进行了实验性传播，分别在5周和4周后导致血内期出现。
