利用免疫电子显微镜观察角蛋白相关蛋白在发育中纤维角质层细胞中的定位
Location of Keratin-associated Proteins in Developing Fiber Cuticle Cells using Immunoelectron Microscopy.
作者信息
Jones Ln, Rogers Ge, Rufaut N, Sinclair Rd
机构信息
Department of Dermatology, University of Melbourne (St. Vincent's Hospital), Fitzroy, Australia.
出版信息
Int J Trichology. 2010 Jul;2(2):89-95. doi: 10.4103/0974-7753.77512.
AIMS
To investigate the location of keratin-associated proteins (KAPs) in developing hair fiber cuticle cells using transmission electron microscopy with immunogold techniques and specific antibodies. Other studies were aimed at detecting the presence of cornified envelope proteins including involucrin and loricrin. MATEIALS AND METHODS: Polyclonal antibodies were produced in rabbits against peptides from KAPS 5.1, KAPS 10.1 ultra high-sulfur proteins.
RESULTS
The KAP proteins were found to form part of the developing exocuticle and a- layer. Cornified envelope proteins (involucrin and loricrin) were absent consistent with recent findings.
CONCLUSIONS
The results have been discussed in terms of a revised model for fiber cuticle surface barriers including their role in fiber cuticle surface function.
目的
运用免疫金技术和特异性抗体的透射电子显微镜，研究角蛋白相关蛋白（KAPs）在发育中的毛发纤维角质形成细胞中的定位。其他研究旨在检测包括兜甲蛋白和loricrin在内的角质包膜蛋白的存在。材料与方法：用兔针对来自KAPS 5.1、KAPS 10.1超高硫蛋白的肽段制备多克隆抗体。
结果
发现KAP蛋白构成发育中外角质层和α层的一部分。与最近的研究结果一致，未检测到角质包膜蛋白（兜甲蛋白和loricrin）。
结论
已根据纤维角质层表面屏障的修订模型对结果进行了讨论，包括它们在纤维角质层表面功能中的作用。
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