Nieves Elsa, Buelvas Neudo, Rondón Maritza, González Néstor
LAPEX-Laboratorio de Parasitología Experimental, Departamento de Biología, Facultad de Ciencias, Universidad de Los Andes, Mérida, Venezuela.
Biomedica. 2010 Jul-Sep;30(3):401-9.
Leishmaniasis is a vector-borne disease transmitted by the intradermal inoculation of Leishmania (Kinetoplastida: Trypanosomatidae) promastigotes together with saliva during the bite of an infected sand fly.
The salivary glands were compared from two vector species, Lutzomyia ovallesi (Ortiz,1952) and Lutzomyia migonei (França,1920) (Diptera: Psychodidae).
Protein profiles by SDS PAGE of salivary glands were compared among species as well as their development at several times post feeding. First, mice were immunized to salivary proteins by exposure to biting by L. ovallesi and of L. migonei. Antibodies in these mice against salivary gland-specific proteins were evaluated by immunoblotting.
No apparent change was revealed in the kinetic expression of salivary proteins induced by the different physiological states post feeding. Qualitative and quantitative variations were detected in16-18 polypeptides with molecular weights ranging from 6 to 180 kDa. Species-specific proteins were demonstrated for L. migonei and L. ovallesi. In addition, antibodies against salivary gland specific proteins were found in mice immunized by the saliva of both species.
Basic information was obtained concerning the nature of salivary gland proteins of L. migonei and L. ovallesi. This information helps to elucidate the role of salivary proteins and their potential as effective tools in screening risk factors in human and other vertebrate hosts.
利什曼病是一种媒介传播疾病，通过受感染的白蛉叮咬时将利什曼原虫（动质体目：锥虫科）前鞭毛体与唾液一起皮内接种传播。
比较两种媒介物种卵形罗蛉（奥尔蒂斯，1952年）和米氏罗蛉（弗朗萨，1920年）（双翅目：毛蠓科）的唾液腺。
比较各物种唾液腺通过SDS-PAGE得到的蛋白质谱及其在进食后不同时间的发育情况。首先，通过让小鼠暴露于卵形罗蛉和米氏罗蛉的叮咬中来使其对唾液蛋白产生免疫。通过免疫印迹法评估这些小鼠体内针对唾液腺特异性蛋白的抗体。
进食后不同生理状态诱导的唾液蛋白动力学表达未显示出明显变化。在分子量范围为6至180 kDa的16 - 18种多肽中检测到定性和定量变化。证明了米氏罗蛉和卵形罗蛉存在物种特异性蛋白。此外，在通过两种物种的唾液免疫的小鼠中发现了针对唾液腺特异性蛋白的抗体。
获得了关于米氏罗蛉和卵形罗蛉唾液腺蛋白性质的基础信息。这些信息有助于阐明唾液蛋白的作用及其作为筛选人类和其他脊椎动物宿主风险因素的有效工具的潜力。
