淀粉样纤维的干燥和水合X射线散射分析。

Dried and hydrated X-ray scattering analysis of amyloid fibrils.

作者信息

Gras Sally L, Squires Adam M

机构信息

Department of Chemistry, The University of Reading, Reading, UK.

出版信息

Methods Mol Biol. 2011;752:147-63. doi: 10.1007/978-1-60327-223-0_10.

DOI:10.1007/978-1-60327-223-0_10

PMID:21713636

Abstract

Wide angle X-ray scattering is a key technique for the analysis of amyloid fibrils that can be used to -confirm the presence of a characteristic cross-beta fibril structure and to characterise the arrangement of beta-strands and beta-sheets within this fibril core. Further structural insight can be obtained by the comparison of X-ray scattering data obtained for dried and hydrated fibril samples. We describe simple techniques for the preparation of dried and hydrated fibril samples for X-ray analysis and the subsequent analysis of X-ray scattering patterns using custom built and readily available software.

摘要

广角X射线散射是分析淀粉样纤维的一项关键技术，可用于确认特征性交叉β纤维结构的存在，并表征该纤维核心内β链和β片层的排列。通过比较干燥和水合纤维样品的X射线散射数据，可以获得进一步的结构信息。我们描述了用于制备干燥和水合纤维样品以进行X射线分析的简单技术，以及使用定制的且易于获得的软件对X射线散射图案进行后续分析的方法。