Slomiany B L, Wang X Y, Palecz D, Okazaki K, Slomiany A
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark.
Alcohol Clin Exp Res. 1990 Aug;14(4):580-3. doi: 10.1111/j.1530-0277.1990.tb01205.x.
The mechanism of gastric mucosal protection by an antiulcer agent, colloidal bismuth subcitrate (CBS), against ethanol-induced injury was investigated using in vivo and in vitro systems. The experiments in vivo were conducted with groups of rats with and without indomethacin pretreatment, and the animals received either a dose of CBS (100 mg/kg) or a vehicle (saline), followed 30 min later by ethanol. In the in vitro studies, gastric mucosa segments were cultured in the presence of CBS, ethanol, or both. The results of in vivo experiments revealed that in the absence of CBS, ethanol caused extensive gastric hemorrhagic lesions which were significantly reduced following CBS pretreatment and this effect of CBS was not prevented by indomethacin. The data obtained with gastric mucosal culture established that in comparison to the controls, ethanol caused a 27% decrease in mucin synthesis, while mucin synthesis in the presence of CBS increased by 48%. The increase in mucin synthesis evoked by CBS was accompanied by the enhanced metabolism of mucosal phosphoinositides, as reflected by a decrease in PI (15%) and PIP2 (30%), and an increase in IP1 (26%) and IP3 (67%). In contrast, ethanol, which exhibited detrimental effect on mucin synthesis, caused a decrease in PIP (35%), IP2 (47%) and IP3 (38%), and an increase in PIP2 (80%), and IP1 (51%). However, when the mucosal culture was carried out in the presence of both CBS and ethanol, the detrimental changes evoked by ethanol on mucin synthesis were prevented, and the phosphoinositide and inositide phosphate distribution patterns were quite similar to those in the mucosa cultured in the presence of CBS only.(ABSTRACT TRUNCATED AT 250 WORDS)
采用体内和体外系统研究了抗溃疡药枸橼酸铋钾（CBS）对乙醇诱导的胃黏膜损伤的保护机制。体内实验对有或无吲哚美辛预处理的大鼠组进行，动物接受一剂CBS（100mg/kg）或赋形剂（生理盐水），30分钟后给予乙醇。体外研究中，胃黏膜段在CBS、乙醇或两者存在的情况下进行培养。体内实验结果显示，在无CBS时，乙醇导致广泛的胃出血性病变，CBS预处理后显著减轻，且吲哚美辛不能阻止CBS的这种作用。胃黏膜培养获得的数据表明，与对照组相比，乙醇使黏蛋白合成减少27%，而在CBS存在时黏蛋白合成增加48%。CBS引起的黏蛋白合成增加伴随着黏膜磷酸肌醇代谢增强，表现为PI（15%）和PIP2（30%）减少，IP1（26%）和IP3（67%）增加。相反，对黏蛋白合成有不利影响的乙醇导致PIP（35%）、IP2（47%）和IP3（38%）减少，PIP2（80%）和IP1（51%）增加。然而，当在CBS和乙醇同时存在的情况下进行黏膜培养时，乙醇引起的对黏蛋白合成的有害变化得到阻止，磷酸肌醇和磷酸肌醇磷酸的分布模式与仅在CBS存在下培养的黏膜中的模式非常相似。（摘要截短至250字）
