Department of Nutrition, University of California, Davis, California 95616, USA.
J Proteome Res. 2011 Aug 5;10(8):3530-41. doi: 10.1021/pr200149t. Epub 2011 Jun 29.
The milk fat globule membrane (MFGM) contains proteins which have been implicated in a variety of health benefits. Milk fat globule membrane proteins were isolated from human milk during a 12 month lactation period and subjected to in-solution digestion and liquid chromatography tandem mass spectrometry analysis. Data were pooled, and our results showed that 191 proteins were identified. Relative quantification of the identified MFGM proteins during the course of lactation was performed by label free spectral counting and differentiation expression analysis, which showed some proteins decreasing during the course of lactation whereas some increased or remained at a relatively constant level. The human MFGM proteins are distributed between intracellular, extracellular, and membrane-associated proteins, and they are mainly involved in cell communication and signal transduction, immune function, metabolism and energy production. This study provides more insights into the dynamic composition of human MFGM proteins, which in turn will enhance our understanding of the physiological significance of MFGM proteins.
牛奶脂肪球膜（MFGM）含有多种具有健康益处的蛋白质。本研究在 12 个月哺乳期内从人乳中分离 MFGM 蛋白，并进行溶液内消化和液相色谱串联质谱分析。对数据进行合并后，共鉴定出 191 种蛋白质。通过无标记谱计数和差异表达分析对哺乳期 MFGM 蛋白进行相对定量，结果显示部分蛋白质在哺乳期逐渐减少，而部分蛋白质增加或保持相对恒定水平。人 MFGM 蛋白分布于细胞内、细胞外和膜相关蛋白中，主要参与细胞通讯和信号转导、免疫功能、代谢和能量产生。本研究深入了解了人 MFGM 蛋白的动态组成，从而进一步加深我们对 MFGM 蛋白生理意义的理解。
