Department of Biology, Selcuk University, Konya, Turkey.
Nat Prod Res. 2012;26(1):1-10. doi: 10.1080/14786419.2010.527839. Epub 2011 Jun 30.
In this study, composition of essential oil and antioxidant capacity of Centaurea drabifolia subsp. detonsa were investigated. The antioxidant capacity of the methanolic extract was evaluated by various methods including measuring the total phenolic content, total antioxidant capacity, free radical scavenging activity (DPPH assay), β-carotene/linoleic acid bleaching assay and ferric and cupric ion reducing power assay. The composition of essential oil was identified by using gas chromatography-mass spectrometry. Totally, 41 compounds were described in the essential oil. Germacrene D (44.829%) was determined as the major compound of the essential oil. The total phenolic content, total antioxidant capacity, inhibition rate of oxidation of linoleic acid, IC(50) (in DPPH assay) and EC(50) (in reducing power) value were found to be 40.454 mg GAE/g, 100.840 mg AAE/g, 65.639%, 39.584 µg mL(-1) and 0.603 mg mL(-1), respectively. The results indicated that the extract of C. drabifolia subsp. detonsa has strong antioxidant properties and this species can be used as a natural antioxidant in food processing and pharmaceutical industries.
本研究调查了刺儿菜亚种钝叶的精油组成和抗氧化能力。采用测定总酚含量、总抗氧化能力、自由基清除活性(DPPH 法)、β-胡萝卜素/亚油酸褪色试验和铁/铜离子还原能力试验等方法评价了甲醇提取物的抗氧化能力。采用气相色谱-质谱联用技术鉴定了精油的组成。精油中共描述了 41 种化合物。鉴定出的主要化合物为大根香叶烯 D(44.829%)。总酚含量、总抗氧化能力、亚油酸氧化抑制率、IC(50)(DPPH 法)和 EC(50)(还原力)值分别为 40.454 mg GAE/g、100.840 mg AAE/g、65.639%、39.584 μg mL(-1)和 0.603 mg mL(-1)。结果表明,刺儿菜亚种钝叶提取物具有较强的抗氧化性能,该物种可在食品加工和制药工业中用作天然抗氧化剂。