单纯疱疹病毒蛋白Vmw65可在神经元细胞中反式激活病毒和细胞启动子。

The herpes simplex virus protein Vmw65 can trans-activate both viral and cellular promoters in neuronal cells.

作者信息

Estridge J K, Kemp L M, Latchman D S

机构信息

Department of Biochemistry, University College and Middlesex School of Medicine, London.

出版信息

Biochem J. 1990 Oct 1;271(1):273-6. doi: 10.1042/bj2710273.

DOI:10.1042/bj2710273

PMID:2171502

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149545/

Abstract

Transcription of the herpes simplex virus (HSV) immediate-early (IE) genes in lytic infection is dependent upon the formation of a complex between the cellular transcription factor Oct-1 and the HSV virion protein Vmw65. This complex then binds to the TAATGARAT sequence in the IE promoters and trans-activates the IE genes. Following infection of neuronal cells such as the C1300 neuroblastoma cell line, however, the viral (IE) genes are not transcribed and the lytic cycle is aborted at an early stage. We show here that the cellular factors necessary to form a trans-activating complex with Vmw65 are present in C1300 cells and that trans-activation of both viral and cellular promoters by Vmw65 can be observed in these cells. In contrast with permissive cells, however, trans-activation is only observed in C1300 cells at a high concentration of the target viral promoter and not at a low concentration of the target promoter, regardless of the amount of Vmw65 transfected. The significance of these effects for the regulation of latent infection and cellular gene expression in neuronal cells is discussed.

摘要

单纯疱疹病毒（HSV）即刻早期（IE）基因在裂解感染中的转录依赖于细胞转录因子Oct-1与HSV病毒体蛋白Vmw65之间形成的复合物。然后该复合物与IE启动子中的TAATGARAT序列结合并反式激活IE基因。然而，在用神经元细胞如C1300神经母细胞瘤细胞系感染后，病毒（IE）基因不被转录，裂解周期在早期阶段中止。我们在此表明，与Vmw65形成反式激活复合物所需的细胞因子存在于C1300细胞中，并且在这些细胞中可以观察到Vmw65对病毒和细胞启动子的反式激活。然而，与允许性细胞不同，无论转染的Vmw65量如何，反式激活仅在高浓度的靶病毒启动子下在C1300细胞中观察到，而在低浓度的靶启动子下则未观察到。讨论了这些效应对于神经元细胞中潜伏感染和细胞基因表达调控的意义。