Augusti-Tocco G, Sato G
Proc Natl Acad Sci U S A. 1969 Sep;64(1):311-5. doi: 10.1073/pnas.64.1.311.
Clonal lines of neurons were obtained in culture from a mouse neuroblastoma. The neuroblastoma cells were adapted to culture growth by the animal-culture alternate passage technique and cloned after single-cell plating. The clonal lines retained the ability to form tumors when injected back into mice. A striking morphological change was observed in the cells adapted to culture growth; they appeared as mature neurons, while the cells of the tumor appeared as immature neuroblasts. Acetylcholinesterase and the enzymes for the synthesis of neurotransmitters, cholineacetylase and tyrosine hydroxylase were assayed in the tumor and compared with brain levels; tyrosine hydroxylase was found to be particularly high, as described previously in human neuroblastomas. The three enzymes were found in the clonal cultures at levels comparable to those found in the tumors. Similarly, there were no remarkable differences between the three clones examined.
从一只小鼠神经母细胞瘤中培养获得了神经元克隆系。通过动物培养交替传代技术使神经母细胞瘤细胞适应培养生长，并在单细胞接种后进行克隆。当将这些克隆系重新注射回小鼠体内时，它们保留了形成肿瘤的能力。在适应培养生长的细胞中观察到了显著的形态变化；它们呈现为成熟神经元，而肿瘤细胞则呈现为未成熟神经母细胞。对肿瘤中的乙酰胆碱酯酶以及神经递质合成酶、胆碱乙酰转移酶和酪氨酸羟化酶进行了测定，并与脑内水平进行比较；发现酪氨酸羟化酶特别高，如先前在人类神经母细胞瘤中所描述的那样。在克隆培养物中发现这三种酶的水平与在肿瘤中发现的水平相当。同样，所检测的三个克隆之间没有显著差异。
