Involvement of pertussis toxin-sensitive GTP-binding protein in prostaglandin F2 alpha- induced phosphoinositide hydrolysis in osteoblast-like cells.

作者信息

Miwa M, Tokuda H, Tsushita K, Kotoyori J, Takahashi Y, Ozaki N, Kozawa O, Oiso Y

机构信息

First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1229-35. doi: 10.1016/0006-291x(90)90817-7.

Prostaglandin F2 alpha (PGF2 alpha) stimulated the formation of inositol phosphates in a dose-dependent manner in cloned osteoblast-like MC3T3-E1 cells. This reaction was markedly inhibited dose-dependently by pertussis toxin. In the cell membranes, pertussis toxin-catalyzed ADP-ribosylation of a 40-kDa protein was significantly attenuated by pretreatment of PGF2 alpha. These results suggest that pertussis toxin-sensitive GTP-binding protein is involved in the coupling of PGF2 alpha receptor to phospholipase C in these cells.