Siddique H R, Sarkar N H
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Biochem Biophys Res Commun. 1990 Oct 15;172(1):348-56. doi: 10.1016/s0006-291x(05)80216-5.
Using polyacrylamide gel mobility shift assay we have detected a nuclear factor (NF-S) in a mouse mammary tumor cell line (GR) that binds to an upstream sequence domain (-766 to -737) near the 5'-end of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR). Antibodies to the products of the Jun and Fos oncogenes interfered with the binding potential of this factor, indicating that the factor shares antigenic determinants with c-Jun/AP-1. In vitro translated c-Jun and c-Fos were also found to bind to the NF-S binding domain consisting of the sequence TGA(A/G)TCA that are known to be recognized by c-Jun/AP-1. Our results raise the possibility that the MMTV-LTR sequence element-766 to -737 by interacting with a Jun/Fos related protein play a role in MMTV transcription and/or the activation of int protooncogenes that are associated with murine mammary tumorigenesis.
利用聚丙烯酰胺凝胶迁移率变动分析，我们在小鼠乳腺肿瘤细胞系（GR）中检测到一种核因子（NF-S），它能与小鼠乳腺肿瘤病毒（MMTV）长末端重复序列（LTR）5'端附近的上游序列结构域（-766至-737）结合。针对Jun和Fos癌基因产物的抗体干扰了该因子的结合能力，表明该因子与c-Jun/AP-1具有共同的抗原决定簇。体外翻译的c-Jun和c-Fos也被发现能与由序列TGA(A/G)TCA组成的NF-S结合结构域结合，已知该序列可被c-Jun/AP-1识别。我们的结果提示，MMTV-LTR序列元件-766至-737通过与Jun/Fos相关蛋白相互作用，可能在MMTV转录和/或与小鼠乳腺肿瘤发生相关的原癌基因激活中发挥作用。
