Chandra M, Poddar M K
Department of Biochemistry, University College of Science, University of Calcutta, India.
Biochem Pharmacol. 1990 Oct 1;40(7):1449-56. doi: 10.1016/0006-2952(90)90439-r.
Aldrin, a chlorinated hydrocarbon, inhibited rat brain synaptosomal membrane-bound Na+,K(+)-adenosine triphosphatase (ATPase) and Mg2(+)-ATPase activities under in vivo and in vitro conditions. Na+,K(+)-ATPase was non-competitively inhibited whereas Mg2(+)-ATPase was inhibited uncompetitively. Arrhenius plots of both these ATPases without aldrin under in vivo and in vitro conditions were found to be linear. In the presence of aldrin, on the other hand, Arrhenius plots of the same ATPases were nonlinear. Slopes of Arrhenius plots of both ATPases under in vivo and in vitro condition were changed with change in temperature with aldrin. The activation energy (AE) of Na+,K(+)-ATPase and Mg2(+)-ATPase activities were changed over the temperature range 15-40 degrees in the presence of aldrin. These results thus suggest that aldrin increases the lipid fluidity of the synaptosomal membrane which may be a cause of inhibition of neuronal membrane-bound Na+,K+ and Mg2(+)-ATPase activities.
艾氏剂，一种氯代烃，在体内和体外条件下均能抑制大鼠脑突触体膜结合的Na⁺,K⁺-腺苷三磷酸酶（ATP酶）和Mg²⁺-ATP酶的活性。Na⁺,K⁺-ATP酶受到非竞争性抑制，而Mg²⁺-ATP酶受到反竞争性抑制。在体内和体外条件下，未添加艾氏剂时这两种ATP酶的阿累尼乌斯曲线均呈线性。另一方面，在有艾氏剂存在的情况下，相同ATP酶的阿累尼乌斯曲线呈非线性。在体内和体外条件下，随着温度变化以及艾氏剂的存在，两种ATP酶的阿累尼乌斯曲线斜率发生改变。在有艾氏剂存在的情况下，在15至40摄氏度的温度范围内，Na⁺,K⁺-ATP酶和Mg²⁺-ATP酶活性的活化能（AE）发生了变化。因此，这些结果表明，艾氏剂增加了突触体膜的脂质流动性，这可能是抑制神经元膜结合的Na⁺,K⁺和Mg²⁺-ATP酶活性的一个原因。
