Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
J Gen Virol. 2011 Oct;92(Pt 10):2446-2457. doi: 10.1099/vir.0.033134-0. Epub 2011 Jun 29.
In the present study, the expression, generation and subcellular localization of Torque teno sus virus (TTSuV) proteins were characterized into two genetically distinct TTSuV species (TTSuV1 and TTSuV2). Following transfection of three TTSuV1 and TTSuV2 full-length ORF (ORF1, ORF2 and ORF3) expression constructs into porcine kidney cells, alternative splice variants encoding new TTSuV protein isoforms were identified for the first time. Proteins encoded from ORF1 and ORF3 were localized in the nucleoli of porcine kidney cells and that of ORF2 in the cytoplasm and nucleus excluding the nucleoli. The subcellular localization of the different protein isoforms was not only similar between distinct TTSuV species but also to the ones described in human Torque teno virus (TTV). Results of the present in vitro study were not based on full-length viral clones but suggested that alternative splicing strategy to generate TTSuV protein isoforms probably occurs in vivo. Obtained data provide new information on molecular biology of TTSuV and anelloviruses, which until now has been solely based on results obtained from human TTV.
在本研究中，Torque teno sus 病毒（TTSuV）蛋白的表达、产生和亚细胞定位被表征为两种具有遗传差异的 TTSuV 种（TTSuV1 和 TTSuV2）。在将三种 TTSuV1 和 TTSuV2 全长 ORF（ORF1、ORF2 和 ORF3）表达构建体转染到猪肾细胞后，首次鉴定出编码新 TTSuV 蛋白同工型的替代剪接变体。ORF1 和 ORF3 编码的蛋白定位于猪肾细胞的核仁中，而 ORF2 则定位于细胞质和细胞核（核仁除外）中。不同蛋白同工型的亚细胞定位不仅在不同的 TTSuV 种之间相似，而且与在人类 Torque teno 病毒（TTV）中描述的相似。本体外研究的结果不是基于全长病毒克隆，但表明替代剪接策略可能在体内产生 TTSuV 蛋白同工型。获得的数据提供了关于 TTSuV 和环状病毒分子生物学的新信息，迄今为止，这些信息仅基于从人类 TTV 获得的结果。
