Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai, India.
J Microbiol Biotechnol. 2011 Jun;21(6):652-8.
The growth kinetics of Streptomyces noursei NRRL 5126 was investigated under different aeration and agitation combinations in a 5.0 l stirred tank fermenter. Poly-epsilon-lysine biosynthesis, cell mass formation, and glycerol utilization rates were affected markedly by both aeration and agitation. An agitation speed of 300 rpm and aeration rate at 2.0 vvm supported better yields of 1,622.81 mg/l with highest specific productivity of 15 mg/l.h. Fermentation kinetics performed under different aeration and agitation conditions showed poly- epsilon-lysine fermentation to be a growth-associated production. A constant DO at 40% in the growth phase and 20% in the production phase increased the poly-epsilon-lysine yield as well as cell mass to their maximum values of 1,992.35 mg/l and 20.73 g/l, respectively. The oxygen transfer rate (OTR), oxygen utilization rate (OUR), and specific oxygen uptake rates (qO2) in the fermentation broth increased in the growth phase and remained unchanged in the stationary phase.
在 5.0 升搅拌罐式发酵罐中，研究了不同通气和搅拌组合对诺尔斯链霉菌 NRRL5126 生长动力学的影响。聚-ε-赖氨酸的生物合成、细胞质量的形成和甘油利用率都明显受到通气和搅拌的影响。在 300rpm 的搅拌速度和 2.0vvm 的通气率下，支持更好的产量为 1622.81mg/l，最高比生产力为 15mg/l.h。在不同通气和搅拌条件下进行的发酵动力学研究表明，聚-ε-赖氨酸发酵是一种与生长相关的生产过程。在生长阶段将 DO 保持在 40%，在生产阶段保持在 20%，可将聚-ε-赖氨酸的产量以及细胞质量提高到最大值，分别为 1992.35mg/l 和 20.73g/l。发酵液中的氧传递速率（OTR）、氧利用率（OUR）和比耗氧速率（qO2）在生长阶段增加，在静止阶段保持不变。
