Felipe A, Moule S K, McGivan J D
Department of Biochemistry, School of Medical Science, University Walk, Bristol, U.K.
Biochim Biophys Acta. 1990 Nov 2;1029(1):61-6. doi: 10.1016/0005-2736(90)90436-r.
The effect of bicarbonate on the uptake of 22Na+ into liver basolateral plasma membrane vesicles was studied under conditions where the pH of the medium was controlled by the use of high buffer concentrations. Bicarbonate stimulated the rate of Na+ uptake only in the presence of a pH gradient (acid inside). The stimulation by bicarbonate was inhibited by both amiloride and DIDS. No evidence for electrogenic Na+/HCO3- symport was found. These results are in part consistent with electroneutral Na+/HCO3- symport, but other explanations cannot be excluded.
在通过使用高缓冲液浓度来控制培养基pH值的条件下，研究了碳酸氢盐对22Na+进入肝基底外侧质膜囊泡摄取的影响。碳酸氢盐仅在存在pH梯度（内部为酸性）的情况下刺激Na+摄取速率。碳酸氢盐的刺激作用被氨氯吡咪和二异丙基氟磷酸（DIDS）抑制。未发现电中性Na+/HCO3-同向转运的证据。这些结果部分与电中性Na+/HCO3-同向转运一致，但不能排除其他解释。
