Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
Arch Microbiol. 2012 Feb;194(2):65-73. doi: 10.1007/s00203-011-0728-3. Epub 2011 Jun 30.
Corynebacterium glutamicum has one RNase E/G ortholog and one RNase J ortholog but no RNase Y. We previously reported that the C. glutamicum NCgl2281 gene encoding the RNase E/G ortholog complemented the rng::cat mutation in Escherichia coli but not the rne-1 mutation. In this study, we constructed an NCgl2281 knockout mutant and found that the mutant cells accumulated 5S rRNA precursor molecules. The processing of 16S and 23S rRNA, tRNA, and tmRNA was normal. Primer extension analysis revealed that the RNase E/G ortholog cleaved at the -1 site of the 5' end of 5S rRNA. However, 3' maturation was essentially unaffected. These findings showed that C. glutamicum NCgl2281 endoribonuclease is involved in the 5' maturation of 5S rRNA. This is the first report showing the physiological function of the RNase E/G ortholog in bacteria having one RNase E/G and one RNase J but no RNase Y.
谷氨酸棒杆菌有一个 RNase E/G 直系同源物和一个 RNase J 直系同源物，但没有 RNase Y。我们之前曾报道过，编码 RNase E/G 直系同源物的谷氨酸棒杆菌 NCgl2281 基因可弥补大肠杆菌中的 rng::cat 突变，但不能弥补 rne-1 突变。在这项研究中，我们构建了一个 NCgl2281 敲除突变体，发现突变细胞积累了 5S rRNA 前体分子。16S 和 23S rRNA、tRNA 和 tmRNA 的加工正常。引物延伸分析表明，RNase E/G 直系同源物在 5S rRNA 5'端的-1 位点切割。然而，3'成熟基本上没有受到影响。这些发现表明，谷氨酸棒杆菌 NCgl2281 内切核酸酶参与 5S rRNA 的 5'成熟。这是第一个表明在具有一个 RNase E/G 和一个 RNase J 但没有 RNase Y 的细菌中，RNase E/G 直系同源物具有生理功能的报告。
