Stamatovic Svetlana M, Keep Richard F, Andjelkovic Anuska V
Methods Mol Biol. 2011;762:303-20. doi: 10.1007/978-1-61779-185-7_22.
Claudin-5 is a transmembrane tight junction protein highly expressed in brain endothelial cells, the site of the blood-brain barrier. The properties of the brain endothelial tight junction complex are considered to be dependent on claudin-5 cell-cell interaction, putting this protein in a position to play a major role in the maintenance of brain endothelial barrier integrity. Thus, alterations in claudin-5 function can lead to "opening" of the paracellular route and increased brain endothelial barrier permeability. Recent work from the authors's laboratory has established that caveolae-dependent internalization/recycling of claudin-5 is a mechanism underlying transient increases in brain endothelial paracellular permeability in the presence of pro-inflammatory mediators. The biochemical and microscopic techniques presented here were used to investigate trafficking of claudin-5 during those changes in paracellular permeability.
Claudin-5是一种跨膜紧密连接蛋白，在脑内皮细胞（血脑屏障所在部位）中高度表达。脑内皮紧密连接复合体的特性被认为依赖于Claudin-5的细胞间相互作用，这使得该蛋白在维持脑内皮屏障完整性方面发挥主要作用。因此，Claudin-5功能的改变可导致细胞旁途径“开放”，并增加脑内皮屏障通透性。作者实验室最近的研究表明，Claudin-5依赖小窝的内化/再循环是在促炎介质存在的情况下脑内皮细胞旁通透性短暂增加的一种机制。本文介绍的生化和显微镜技术用于研究细胞旁通透性变化期间Claudin-5的转运情况。
