Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, Zhejiang Province, PR China.
Fish Shellfish Immunol. 2011 Sep;31(3):439-45. doi: 10.1016/j.fsi.2011.06.016. Epub 2011 Jun 21.
Chemokines are a large family of chemotactic cytokines playing crucial roles in the innate immune response. In the present study, we report the cloning of a CXC chemokine gene resembling the closely related CXCL9/CXCL10/CXCL11 from the miiuy croaker Miichthys miiuy (MimiCXC). Both 5'-RACE and 3'-RACE were carried out in order to obtain the complete cDNA, which consists of a 73 bp 5'-UTR, a 369 bp open reading frame encoding 122 amino acids and a 715 bp 3'-UTR. The deduced MimiCXC contains a 19-aa signal peptide and a 103-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known CXC chemokines. It shares 4.8%-65.6% sequence identities to mammalian CXC chemokines and the highest sequence identity of 65.6% is between MimiCXC and CXCL10 chemokine. Three exons and two introns were identified in MimiCXC gene. The MimiCXC gene was constitutively expressed in all tissues tested, although at different levels. Upon induction with Vibrio anguillarum, MimiCXC gene expression was up-regulated in kidney and spleen, however, down-regulated in liver. These results indicate that MimiCXC may be involved in immune responses as well as homeostatic processes in miiuy croaker.
趋化因子是一类重要的趋化细胞因子家族，在先天免疫反应中发挥着关键作用。本研究报道了从米氏绒螯蟹（Miichthys miiuy）中克隆的一个 CXC 趋化因子基因，该基因类似于密切相关的 CXCL9/CXCL10/CXCL11（MimiCXC）。为了获得完整的 cDNA，进行了 5'-RACE 和 3'-RACE，该 cDNA 由 73bp 的 5'-UTR、369bp 的开放阅读框编码 122 个氨基酸和 715bp 的 3'-UTR 组成。推导的 MimiCXC 包含一个 19 个氨基酸的信号肽和一个 103 个氨基酸的成熟多肽，其具有其他已知 CXC 趋化因子中发现的典型四个半胱氨酸排列。它与哺乳动物 CXC 趋化因子的序列同一性为 4.8%-65.6%，与 CXCL10 趋化因子的最高序列同一性为 65.6%。在 MimiCXC 基因中鉴定出三个外显子和两个内含子。MimiCXC 基因在所有检测的组织中均呈组成型表达，尽管表达水平不同。在鳗弧菌诱导后，MimiCXC 基因在肾脏和脾脏中表达上调，但在肝脏中表达下调。这些结果表明，MimiCXC 可能参与米氏绒螯蟹的免疫反应和稳态过程。
