Department of Microbiology, University of Occupational and Environmental Health, Japan.
Biol Pharm Bull. 2011;34(7):1011-20. doi: 10.1248/bpb.34.1011.
Intestinal microbiotas of human subjects and effect of antibiotic treatment on them have been reported with cultivation independent methods. However, Japanese fecal microbiotas have not been studied enough. We have constructed a clone library method to obtain results within 3 d. In this study, intestinal microbiotas of 29 healthy Japanese adults, whose fecal samples were collected twice at 5 month intervals from each subject, were analyzed with our clone library method, and using those data as a benchmark effect of antibiotic treatment on intestinal microbiotas was evaluated. The fifty-eight fecal microbiotas were assessed based on percentages at genus level, and the variability was analyzed with a principal component analysis (PCA). PCA showed that the microbiotas divided into three groups depending on the large eigenvectors (genera Ruminococcus, Bacteroides, and Prevotella), and the dual samples from the twenty-two individuals have belonged to the same PCA group. It suggests that almost Japanese adults have own stable intestinal microbiota. The genera Ruminococcus and Bacteroides were present in almost subjects, while the genus Prevotella was found only in nine subjects (approximately 30%) which was preserved with 5 months intervals. Next, the microbiotas before and after antibiotic treatment were evaluated comparing with the 58 healthy adult microbiotas. The results showed that beta-lactams influenced profoundly on intestinal microbiotas and the effect of macrolides depended on the cases. It suggests that our clone library method could show overview of intestinal microbiota and would give us useful information about the effect of antibiotic treatment for daily clinical diagnosis.
采用培养依赖方法已经报道了人类肠道微生物群及其抗生素治疗效果。然而，日本粪便微生物群还没有得到充分的研究。我们构建了一种克隆文库方法，可在 3 天内获得结果。在这项研究中，我们使用我们的克隆文库方法分析了 29 名健康日本成年人的肠道微生物群，这些成年人的粪便样本每隔 5 个月从每个对象中收集两次，并利用这些数据评估抗生素治疗对肠道微生物群的影响。基于属水平的百分比评估了 58 个粪便微生物群，并使用主成分分析（PCA）进行了变异分析。PCA 表明，微生物群根据大特征向量（属罗伊氏乳杆菌、拟杆菌和普雷沃氏菌）分为三组，22 名个体的双重样本属于同一 PCA 组。这表明，几乎所有日本成年人都有自己稳定的肠道微生物群。属罗伊氏乳杆菌和拟杆菌几乎存在于所有对象中，而属普雷沃氏菌仅存在于 9 名（约 30%）个体中，这些个体在 5 个月的间隔内被保留下来。接下来，通过比较 58 名健康成年人的微生物群，评估了抗生素治疗前后的微生物群。结果表明，β-内酰胺类抗生素对肠道微生物群有很大影响，大环内酯类抗生素的效果取决于具体情况。这表明，我们的克隆文库方法可以显示肠道微生物群的全貌，并为我们提供有关抗生素治疗对日常临床诊断影响的有用信息。
